A randomised controlled trial of increasing fruit and vegetable intake and how this influences the carotenoid concentration and activities of PON-1 and LCAT in HDL from subjects with type 2 diabetes.
Study Goal
The researchers aimed to determine if increased fruit and vegetable (F&V) intake, which includes lutein, influenced the carotenoid content and antioxidant-related enzymes in HDL in subjects with type 2 diabetes (T2D).
Results Summary
The study found that increased F&V intake significantly elevated lutein levels in serum, HDL2, and HDL3 (p = 0.012), alongside enhanced activity of antioxidant enzymes like PON-1 and LCAT in HDL3. These changes suggest improved cardioprotective properties of HDL.
Population
Eighty obese subjects with type 2 diabetes.
Effective Dosage
≥6 portions/day of fruits and vegetables (specific lutein dosage not isolated).
Duration
8 weeks.
Interactions
None mentioned.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
≥6-portion/day fruit and vegetable (F&V) diet | increase | α-carotene in serum, HDL2 and HDL3 | obese subjects with type 2 diabetes (T2D) | p = 0.008 | increased | #1 |
≥6-portion/day fruit and vegetable (F&V) diet | increase | β-cryptoxanthin in serum, HDL2 and HDL3 | obese subjects with type 2 diabetes (T2D) | p = 0.042 | increased | #2 |
≥6-portion/day fruit and vegetable (F&V) diet | increase | lutein in serum, HDL2 and HDL3 | obese subjects with type 2 diabetes (T2D) | p = 0.012 | increased | #3 |
≥6-portion/day fruit and vegetable (F&V) diet | increase | lycopene in serum, HDL2 and HDL3 | obese subjects with type 2 diabetes (T2D) | p = 0.016 | increased | #4 |
≥6-portion/day fruit and vegetable (F&V) diet | increase | activity of paraoxonase-1 (PON-1) in HDL3 | obese subjects with type 2 diabetes (T2D) | p = 0.006 | increased | #5 |
≥6-portion/day fruit and vegetable (F&V) diet | increase | activity of lecithin cholesterol acyltransferase (LCAT) in HDL3 | obese subjects with type 2 diabetes (T2D) | p = 0.044 | increased | #6 |
increased fruit and vegetable (F&V) intake | increase | carotenoid content of HDL | subjects with T2D | - | augmented | #7 |
increased fruit and vegetable (F&V) intake | increase | enzymes associated with the antioxidant properties of HDL | subjects with T2D | - | influenced | #8 |
BACKGROUND: High density lipoproteins (HDL) have many cardioprotective roles; however, in subjects with type 2 diabetes (T2D) these cardioprotective properties are diminished. Conversely, increased fruit and vegetable (F&V) intake may reduce cardiovascular disease risk, although direct trial evidence of a mechanism by which this occurs in subjects with T2D is lacking. Therefore, the aim of this study was to examine if increased F&V consumption influenced the carotenoid content and enzymes associated with the antioxidant properties of HDL in subjects with T2D. METHODS: Eighty obese subjects with T2D were randomised to a 1- or ≥6-portion/day F&V diet for 8-weeks. Fasting serum was collected pre- and post-intervention. HDL was subfractionated into HDL2 and HDL3 by rapid ultracentrifugation. Carotenoids were measured in serum, HDL2 and HDL3 by high performance liquid chromatography. The activity of paraoxonase-1 (PON-1) was measured in serum, HDL2 and HDL3 by a spectrophotometric assay, while the activity of lecithin cholesterol acyltransferase (LCAT) was measured in serum, HDL2 and HDL3 by a fluorometric assay. RESULTS: In the ≥6- vs. 1-portion post-intervention comparisons, carotenoids increased in serum, HDL2 and particularly HDL3, (α-carotene, p = 0.008; β-cryptoxanthin, p = 0.042; lutein, p = 0.012; lycopene, p = 0.016), as did the activities of PON-1 and LCAT in HDL3 (p = 0.006 and 0.044, respectively). CONCLUSION: To our knowledge, this is the first study in subjects with T2D to demonstrate that increased F&V intake augmented the carotenoid content and influenced enzymes associated with the antioxidant properties of HDL. We suggest that these changes would enhance the cardioprotective properties of this lipoprotein. CLINICAL TRIAL REGISTRATION: ISRCTN21676269.