In Vitro Antioxidant and In Vivo Hypolipidemic Effects of the King Oyster Culinary-Medicinal Mushroom, Pleurotus eryngii var. ferulae DDL01 (Agaricomycetes), in Rats with High-Fat Diet-Induced Fatty Liver and Hyperlipidemia.
Study Goal
The researchers aimed to determine whether Pleurotus eryngii var. ferulae DDL01 (King Oyster) could reduce oxidative damage and improve lipid profiles in a high-fat/high-cholesterol-induced hyperlipidemic rat model.
Results Summary
The study found that King Oyster extracts exhibited strong antioxidant activity, reduced harmful lipid markers, and increased beneficial HDL cholesterol in hyperlipidemic rats. Limitations include the use of an animal model, which may not fully translate to human effects.
Population
Hyperlipidemic rat model (no human subjects).
Effective Dosage
7.5 g/rat/day of pellet diet containing King Oyster.
Duration
3 weeks.
Interactions
None mentioned.
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
water extracts of the fruiting bodies of Pleurotus eryngii var. ferulae DDL01 | decrease | DPPH radicals | in vitro studies | 139.46 ± 3.2 μg | showed strong scavenging activities | #1 |
water extracts of the fruiting bodies of Pleurotus eryngii var. ferulae DDL01 | decrease | hydroxyl radicals | in vitro studies | 139.46 ± 3.2 μg | showed strong scavenging activities | #2 |
water extracts of the fruiting bodies of Pleurotus eryngii var. ferulae DDL01 | neutral | - | in vitro studies | - | showed Fe2+ chelating and reducing abilities | #3 |
water extracts of the fruiting bodies of Pleurotus eryngii var. ferulae DDL01 | increase | polyphenols | in vitro studies | - | showed a large amount of polyphenols | #4 |
water extracts of the fruiting bodies of Pleurotus eryngii var. ferulae DDL01 | decrease | lipid peroxidation in the liver and brain tissues | in vitro studies | - | showed an inhibitory effect | #5 |
pellet diet containing P. eryngii var. ferulae DDL01 (PD) | decrease | levels of serum triacylglycerols | high-fat/high-cholesterol-induced hyperlipidemic rat model | P < 0.05 | caused a significant decrease | #6 |
pellet diet containing P. eryngii var. ferulae DDL01 (PD) | decrease | levels of low-density lipoprotein cholesterol | high-fat/high-cholesterol-induced hyperlipidemic rat model | P < 0.05 | caused a significant decrease | #7 |
pellet diet containing P. eryngii var. ferulae DDL01 (PD) | decrease | levels of very-low-density lipoprotein cholesterol | high-fat/high-cholesterol-induced hyperlipidemic rat model | P < 0.05 | caused a significant decrease | #8 |
pellet diet containing P. eryngii var. ferulae DDL01 (PD) | decrease | levels of aspartate aminotransferase | high-fat/high-cholesterol-induced hyperlipidemic rat model | P < 0.05 | caused a significant decrease | #9 |
pellet diet containing P. eryngii var. ferulae DDL01 (PD) | decrease | levels of alanine aminotransferase | high-fat/high-cholesterol-induced hyperlipidemic rat model | P < 0.05 | caused a significant decrease | #10 |
pellet diet containing P. eryngii var. ferulae DDL01 (PD) | increase | level of high-density lipoprotein cholesterol | high-fat/high-cholesterol-induced hyperlipidemic rat model | P < 0.05 | caused a significant increase | #11 |
PD administration | decrease | high-fat/high-cholesterol-induced hepatic lipid accumulation | high-fat/high-cholesterol-induced hyperlipidemic rat model | - | significantly decreased | #12 |
Treatment with the extracts (up to 500 μg/mL) | no change | viability of HepG2 and 3T3-L1 cells | in vitro cell cultures | - | did not significantly affect | #13 |
We investigated the effect of the culinary-medicinal mushroom Pleurotus eryngii var. ferulae DDL01 on oxidative damage in the liver and brain and a high-fat/high-cholesterol-induced hyperlipidemic model. In in vitro studies, the water extracts of the fruiting bodies showed strong scavenging activities of DPPH (139.46 ± 3.2 μg) and hydroxyl (139.46 ± 3.2 μg) radicals. Moreover, the extracts showed Fe2+ chelating and reducing abilities, as well as a large amount of polyphenols and an inhibitory effect on lipid peroxidation in the liver and brain tissues. The rats were fed a pellet diet (7.5 g/rat/day) containing P. eryngii var. ferulae DDL01 (PD) for 3 weeks. In the high-fat/high-cholesterol-induced hyperlipidemic rat model, administration of PD caused a significant decrease (P < 0.05) in the levels of serum triacylglycerols, low-density lipoprotein cholesterol, very-low-density lipoprotein cholesterol, aspartate aminotransferase, and alanine aminotransferase and a significant increase (P < 0.05) in the level of high-density lipoprotein cholesterol. PD administration significantly decreased high-fat/high-cholesterol-induced hepatic lipid accumulation. Treatment with the extracts (up to 500 μg/mL) did not significantly affect the viability of HepG2 and 3T3-L1 cells. Our findings suggest that this mushroom has potential as an antiatherogenic dietary source in the development of therapeutic agents and functional foods.