Use of tannase-producing bacteria isolated from the rumen to improve the nutritional value of pomegranate peel for fattening lambs.
Study Goal
The researchers aimed to determine whether treating pomegranate peel with tannase-producing bacteria could reduce its adverse effects (e.g., decreased nutrient intake) and improve its nutritional value for fattening lambs.
Results Summary
Treating pomegranate peel with tannase-producing bacteria improved nutrient intake and digestibility compared to untreated peel, though digestibility remained lower than the control diet. The treatment also restored rumen pH, ammonia nitrogen concentration, and protozoa population, which were negatively affected by untreated peel.
Population
Arabi male lambs (average weight 35 ± 3.8 kg, age 8 ± 1.0 months)
Effective Dosage
Not specified
Duration
Not specified
Interactions
None mentioned
| Intervention | Direction | Endpoint | Population | Dosage | Impact | Claim # |
|---|---|---|---|---|---|---|
diet containing untreated pomegranate peel (raw PP, UTPP) | decrease | nutrient intake | Arabi male lambs | - | decreased | #1 |
diet containing PP treated with tannase-producing bacteria (bacteria treating PP, BTPP) | increase | nutrient intake | Arabi male lambs | - | significantly increased | #2 |
diet containing untreated pomegranate peel (raw PP, UTPP) | decrease | pH of the rumen | Arabi male lambs | - | significantly decreased | #3 |
diet containing untreated pomegranate peel (raw PP, UTPP) | decrease | ammonia nitrogen concentration of the rumen | Arabi male lambs | - | significantly decreased | #4 |
diet containing untreated pomegranate peel (raw PP, UTPP) | decrease | total protozoa population of the rumen | Arabi male lambs | - | significantly decreased | #5 |
diet containing PP treated with tannase-producing bacteria (bacteria treating PP, BTPP) | increase | pH of the rumen | Arabi male lambs | - | increased | #6 |
diet containing PP treated with tannase-producing bacteria (bacteria treating PP, BTPP) | increase | ammonia nitrogen concentration of the rumen | Arabi male lambs | - | increased | #7 |
diet containing PP treated with tannase-producing bacteria (bacteria treating PP, BTPP) | increase | total protozoa population of the rumen | Arabi male lambs | - | increased | #8 |
diet containing untreated pomegranate peel (raw PP, UTPP) | increase | blood glucose | Arabi male lambs | - | had the highest concentration | #9 |
control diet (CNT, no PP) | increase | blood urea nitrogen | Arabi male lambs | - | had the highest concentrations | #10 |
control diet (CNT, no PP) | increase | cholesterol | Arabi male lambs | - | had the highest concentrations | #11 |
control diet (CNT, no PP) | increase | triglyceride | Arabi male lambs | - | had the highest concentrations | #12 |
control diet (CNT, no PP) | increase | low-density lipoprotein | Arabi male lambs | - | had the highest concentrations | #13 |
experimental treatments | increase | dry matter consumption of the whole period | Arabi male lambs | - | was significant | #14 |
experimental treatments | no change | average daily gain | Arabi male lambs | - | no significant effect | #15 |
experimental treatments | no change | feed conversion ratio | Arabi male lambs | - | no significant effect | #16 |
experimental treatments | no change | feed efficiency | Arabi male lambs | - | no significant effect | #17 |
experimental treatments | no change | longissimus muscle colorimetric systems | Arabi male lambs | - | no significant effect | #18 |
BACKGROUND: The use of plants and by-products, which are containing a high amount of secondary and anti-nutritional compounds such as tannins, in animal feed is limited. The methods that can reduce these compounds make facilitate their use in animal feed. OBJECTIVES: The aim of this study was to reduce the adverse effects of pomegranate peel (PP) tannin for fattening lambs using the tannase-producing bacteria. METHODS: Twenty-one Arabi male lambs (averagely 35 ± 3.8 kg weight and 8 ± 1.0 months age) were used in a completely randomized design with three treatments and seven replications in the present experiment. The experimental treatments included 1 - control diet (CNT, no PP), 2 - diet containing untreated PP (raw PP, UTPP) and 3 - diet containing PP treated with tannase-producing bacteria (bacteria treating PP, BTPP). RESULTS: Using UTPP decreased nutrient intake compared to the control and treatment with tannase-producing bacteria again significantly increased nutrient intake compared to the UTPP (p < 0.05). The digestibilities of organic matter, neutral detergent fibre and acid detergent fibre in the control treatment were significantly higher than UTPP and BTPP and in the BTPP were significantly higher than the UTPP (p < 0.05). The use of UTPP in the diet significantly decreased the pH, ammonia nitrogen concentration and the total protozoa population of the rumen compared to the control (p < 0.05), and treatment with bacteria increased them again. The lowest total protozoa population was observed in UTPP treatments (p < 0.05). The highest concentration of blood glucose was observed in UTPP; however, the highest concentrations of blood urea nitrogen, cholesterol, triglyceride, high-density lipoprotein (non-significant) and low-density lipoprotein were in the control treatment. The effect of experimental treatments on the dry matter consumption of the whole period was significant; however, there was no significant effect on average daily gain, feed conversion ratio, feed efficiency and longissimus muscle colorimetric systems. CONCLUSIONS: Therefore, considering the positive effects of treatment PP with tannin-degrading bacteria relative to raw PP, using these bacteria is a proper way to reduce tannin, thus improving the nutritional value of PP for ruminants.